Laboratory Manual for Exercise Physiology

Laboratory 1. Primary Data Collection
Test Variables
Measurement Terminology
Metric Conversions
Background and Environmental Information
Descriptive Statistics
Presentation of Results
Interpretation of Data
Poster Presentations
Laboratory Activity 1.1 Basic Data
Laboratory Activity 1.2 Statistical Procedures
Laboratory Activity 1.3 Tables and Graphs
Laboratory Activity 1.4 Creating a Poster Presentation

Laboratory 2. Pretest Screening
Informed Consent
Physical Activity Readiness Questionnaire for Everyone
Health History Questionnaire
Signs and Symptoms of Disease and Medical Clearance
Coronary Risk Factor Analysis
Lifestyle Evaluation
Disease Risk Stratification
Laboratory Activity 2.1 Basic Screening Procedures
Laboratory Activity 2.2 Pretest Results

Laboratory 3. Flexibility Testing
Direct and Indirect ROM Assessment
Body Areas
Beighton Scoring System
Laboratory Activity 3.1 Traditional, Wall, V-Sit, and Chair Sit-and-Reach Test Comparisons
Laboratory Activity 3.2 YMCA, Backsaver, and Goniometer Test Comparisons
Laboratory Activity 3.3 Canadian, Traditional, and Backsaver Sit-and-Reach Test Comparisons
Laboratory Activity 3.4 Shoulder Flexibility Test Comparisons
Laboratory Activity 3.5 Measuring Ankle Dorsiflexion Range of Motion (ROM)
Laboratory Activity 3.6 Beighton Scoring System Test

Laboratory 4. Blood Pressure Measurements
Blood Pressure Responses to Exercise
Accurate Blood Pressure Checks
Blood Pressure Devices
Laboratory Activity 4.1 Effects of Body Position on Blood Pressure
Laboratory Activity 4.2 Effects of Dynamic Exercise on Blood Pressure
Laboratory Activity 4.3 Effects of Isometric Contractions on Blood Pressure

Laboratory 5. Resting Metabolic Rate Determinations
Aerobic Metabolism and Respiratory Exchange Ratio
Total Energy Expenditure and Resting Metabolic Rate (RMR)
Laboratory Activity 5.1 Estimating Resting Metabolic Rate (RMR)
Laboratory Activity 5.2 Measuring Resting Metabolic Rate (RMR)

Laboratory 6. Oxygen Deficit and EPOC Evaluations
Transition From Rest to Exercise
Oxygen Uptake During Exercise and Recovery
Laboratory Activity 6.1 Calculation of Oxygen Deficit and EPOC on a Treadmill
Laboratory Activity 6.2 Calculation of Oxygen Deficit and EPOC on a Bike
Laboratory Activity 6.3 EPOC Following a Wingate Test

Laboratory 7. Submaximal Exercise Testing
Measurement of Heart Rate
Rating of Perceived Exertion
Laboratory Activity 7.1 Submaximal Bench Step Test
Laboratory Activity 7.2 Submaximal Treadmill Test
Laboratory Activity 7.3 Submaximal Cycle Ergometer Test

Laboratory 8. Aerobic Power Field Assessments
Cooper 1.5-Mile Run/Walk Test
Cooper 12-Minute Run/Walk Test
Rockport Fitness Walking Test
6-Minute Walk Test (6MWT)
Laboratory Activity 8.1 Cooper 1.5-Mile Run/Walk Test and 12-Minute Run/Walk Test
Laboratory Activity 8.2 Rockport Fitness Walking Test
Laboratory Activity 8.3 6-Minute Walking Test (6MWT)

Laboratory 9. High-Intensity Fitness Testing
Léger 20 m Shuttle Run Test
Yo-Yo Intermittent Recovery Test
30-15 Intermittent Fitness Test
Laboratory Activity 9.1 Léger 20 m Shuttle Run Test (20mSRT)
Laboratory Activity 9.2 Yo-Yo Intermittent Recovery Test (Yo-Yo IRT)
Laboratory Activity 9.3 30-15 Intermittent Fitness Test (30-15IFT-40m)
Laboratory Activity 9.4 Modified 30-15 Intermittent Fitness Test (30-15IFT-28m)

Laboratory 10. Maximal Oxygen Consumption Measurements
Selecting a Test Protocol
Monitoring Progress With RPE Scales
Estimating Fuel Usage With RER
Ventilatory Threshold
Laboratory Activity 10.1 Graded Treadmill VO2max Test
Laboratory Activity 10.2 Cycle Ergometer VO2max Test

Laboratory 11. Blood Lactate Threshold Assessment
Determining the Deflection Point
Selecting a Test Method
Role of the Ventilatory Threshold
Laboratory Activity 11.1 Blood Lactate Measurement at Rest
Laboratory Activity 11.2 LT During an Incremental Cycle Test
Laboratory Activity 11.3 Blood Lactate After Anaerobic Exercise

Laboratory 12. Musculoskeletal Fitness Measurements
Assessments of Muscular Strength
Assessments of Muscular Endurance
Laboratory Activity 12.1 Maximal Upper-Body Strength
Laboratory Activity 12.2 Creating a Load-Velocity Profile and Predicting 1RM
Laboratory Activity 12.3 Maximal Lower-Body Strength
Laboratory Activity 12.4 Maximal Handgrip Strength
Laboratory Activity 12.5 Upper-Body Muscular Endurance

Laboratory 13. Anaerobic Fitness Measurements
Sprinting Performance Tests for Estimating Horizontal Power
Jumping Performance Tests for Determining Vertical Power
Formulas for Estimating Vertical Power
Testing Interlimb Asymmetries With Bilateral and Unilateral Jump Tests
Bosco Test for Estimating Power Endurance
Determining the Eccentric Utilization Ratio
Wingate Anaerobic Test for Determining Anaerobic Cycling Power
Margaria-Kalamen Stair-Climb Test for Determining Anaerobic Power
Laboratory Activity 13.1 Sprinting Performance
Laboratory Activity 13.2 Jumping Performance
Laboratory Activity 13.3 Jumping Performance With a Switch Mat
Laboratory Activity 13.4 Jump Performance: Comparing a Smartphone App With a Switch Mat
Laboratory Activity 13.5 Determining Asymmetries With a Force Plate
Laboratory Activity 13.6 Power Endurance
Laboratory Activity 13.7 Anaerobic Cycling Power
Laboratory Activity 13.8 Margaria-Kalamen Stair-Climb Test

Laboratory 14. Pulmonary Function Testing
Pulmonary Function Testing
Pulmonary Function Testing as a Tool for Diagnosing Pulmonary Disease
Respiratory Limitations on Exercise
Laboratory Activity 14.1 Lung Volumes and Capacities
Laboratory Activity 14.2 Pulmonary Function
Laboratory Activity 14.3 Exercise-Induced Ventilatory Limitations
Laboratory Activity 14.4 Exercise-Induced Asthma

Laboratory 15. Body Composition Assessments
Body Composition Models
BMI for Categorizing Health Risk
Circumference Measurements and Health Risk
Skinfold Thickness as a Measure of Body Fat
Laboratory Activity 15.1 BMI and Circumference Data
Laboratory Activity 15.2 Bioelectrical Impedance
Laboratory Activity 15.3 Techniques for Measuring Skinfold Thickness
Laboratory Activity 15.4 Estimating Relative Body Fat Using Hydrodensitometry

Laboratory 16. Electrocardiograph Measurements
Electrical Activity of the Heart
Placement of ECG Leads
Interpreting the ECG Recording
ECG as a Tool for Diagnosing Cardiac Abnormalities
Heart Rate Response to Exercise
Laboratory Activity 16.1 Resting ECG
Laboratory Activity 16.2 Effects of Body Position on the Heart Axis
Laboratory Activity 16.3 Submaximal Exercise Effects With a 12-Lead ECG

Appendix A: Units of Measure Conversions
Appendix B: Estimation of the O2 Cost of Walking, Running, and Leg Ergometry
Appendix C: Haldane Transformation
Appendix D: Metabolic Cart Information
Appendix E: Calibration of Equipment